If you get littermates it shouldn't matter. In my experience with a rescue
group the boys 'in general' are more outgoing and affectionate even after
neutering.

Watch them for a while and choose the two who tend to be close to each other
already and ask their owner too.
I think - but may be wrong - that a male and a female should work well. Two
of the same sex might end up fighting.
But then again I suggest you pick a couple of buddies.

Carola

We've always had a male and a female. I currently have two females and a
male and the females avoid each other like the plague. Our little male can
hang out with both with no problems.
That said if they are littermates it shouldn't make much difference what sex
they are.

My old female has outlived 3 male cats. She always acts like she misses them
when they die but still gives a new one a run for his money until they
adjust to each other.

W

I'm looking forward to getting two kittens someday & my thinking is to get
them from the same litter so they are accustomed to each other.  Is there
any advantage of choosing females or males?  Is there liable to be more
fighting among one female & one male? (They'll both be neutered)
Thanks

Personally, I think males are better pets.  But observe the litter and
pick two kittens who play well together - they will tell you who thir
best friend is.

-L.

Thanks for everyone's replies. Our first cat was a stray who adopted us
about 12 years ago & we were very lucky in that his temperament was as good
as you can expect.  He's been a loner all his life & although we would like
to have another one to keep him company, I feel that it would run him ragged
in his older years.

I just saw this posting so I thought I'd toss my two cents worth.

A cupla points I would add.  As for fighting, I don't think any two cats
who grow up from being kittens are likely to ever really fight.  Have
disagreemenets?  Work it out with a little arm wrestling?  sure.  But
fight, I know its possible, but I think it is pretty unusual.

As for getting two from the same litter, I know a lot of people suggest
that, and it has its points.  But, we got our kittens from two  
different litters and I am glad we did.  They are so different, They
have totally different charcters, personalities, behaviour patterns,
whatever.  They are a constant study to see how they react differently.  
And they get along great.  Squabbles and wrestling matches for sure, but
all in all, they get along at least as well as my wife and I.

Old joke:  We've been happily married for 17 years.  Not bad out of 32.

Minus